Manipulating the Mouse Embryo

Manipulating the Mouse Embryo PDF

Author: Richard Behringer

Publisher:

Published: 2014

Total Pages: 0

ISBN-13: 9781936113019

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Enthält: Genetics and embryology of the mouse: past, present, and future. Summary of mouse development. A mouse colony for the production of transgenic and chimeric animals. Recovery and in vitro culture of preimplantation embryos. Isolation, culture, and manipulation of postimplantation embryos. Surgical procedures. Production of transgenic mice by pronuclear microinjection. Embryo-derived stem cell lines. Germ line-competent stem cells derived from adult mice. Vector designs for pluripotent stem cell-based transgenesis and genome alterations. Introduction of foreign DNA into embryonic stem cells. Production of chimeras. Genotyping. Parthenogenesis, pronuclear transfer, and mouse cloning. Assisted reproduction: ovary transplantation, in vitro fertilization, artificial insemination, and intracytoplasmic sperm injection. Cryopreservation, rederivation, and transport of mouse strains. Techniques for visualizing gene products, cells, tissues, and organ systems. Setting up a micromanipulation lab.

Manipulating the Mouse Embryo

Manipulating the Mouse Embryo PDF

Author: Brigid Hogan

Publisher: Cold Spring Harbor Laboratory Press

Published: 1986

Total Pages: 352

ISBN-13:

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Of mouse development -- Setting up a colony for the production of transgenic mice -- Recovery, culture, and transfer of embryos -- Introduction of new genetic information into the developing mouse embryo -- Iolation of pluripotential stem cell lines -- Techhniques for visualizing genes and gene products -- In vitro culture of eggs, embryos, and teratocarcinoma cels -- Chemicals, supplies, and solutions.

小鼠胚胎操作实验指南

小鼠胚胎操作实验指南 PDF

Author: A.·纳吉 (加)

Publisher:

Published: 2003

Total Pages: 764

ISBN-13: 9787030124388

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本书其余责任者还有:(加)M. 格斯滕斯坦、(德)K. 文特斯滕、(美)R. 贝林杰。

Manipulating the Mouse Embryo

Manipulating the Mouse Embryo PDF

Author: Andras Nagy

Publisher: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press

Published: 2003

Total Pages: 784

ISBN-13:

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Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.

Antibodies

Antibodies PDF

Author: Edward Harlow

Publisher: CSHL Press

Published: 1988

Total Pages: 748

ISBN-13: 9780879693145

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Introduction to immunochemistry for molecular biologists and other nonspecialists. Spiral.

Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research

Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research PDF

Author: National Research Council

Publisher: National Academies Press

Published: 2003-08-22

Total Pages: 223

ISBN-13: 030916785X

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Expanding on the National Research Council's Guide for the Care and Use of Laboratory Animals, this book deals specifically with mammals in neuroscience and behavioral research laboratories. It offers flexible guidelines for the care of these animals, and guidance on adapting these guidelines to various situations without hindering the research process. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research offers a more in-depth treatment of concerns specific to these disciplines than any previous guide on animal care and use. It treats on such important subjects as: The important role that the researcher and veterinarian play in developing animal protocols. Methods for assessing and ensuring an animal's well-being. General animal-care elements as they apply to neuroscience and behavioral research, and common animal welfare challenges this research can pose. The use of professional judgment and careful interpretation of regulations and guidelines to develop performance standards ensuring animal well-being and high-quality research. Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research treats the development and evaluation of animal-use protocols as a decision-making process, not just a decision. To this end, it presents the most current, in-depth information about the best practices for animal care and use, as they pertain to the intricacies of neuroscience and behavioral research.